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目的 探讨端粒酶活性与pINK4b(p15)基因表达在儿童急性白血病(AL)发病中的关系。方法 采用改良端粒重复序列PCR扩增和逆转录聚合酶链反应检测27例儿童AL骨髓单个核细胞端粒酶活性和p15基因表达情况,并与9例正常骨髓单个核细胞端粒酶活性及p15基因表达进行比较。结果 初诊时,AL患儿骨髓细胞端粒酶活性(34. 5±37. 0)TPG较对照组(2. 4±2. 2 )TPG明显增高(P<0. 001 ); AL患儿p15基因表达水平(9. 8±16. 2)%, 明显低于对照组(45. 8±16. 9)% (P<0. 001 )。AL患儿骨髓细胞端粒酶活性与p15基因表达水平之间无相关关系(r= 0.01304, P> 0. 05)。结论 端粒酶活化和p15基因表达水平降低与急性髓性白血病发展有关,但两者可能通过不同的机制作用于白血病。
Objective To investigate the relationship between telomerase activity and pINK4b (p15) gene expression in the pathogenesis of childhood acute leukemia (AL). Methods The telomerase activity and p15 gene expression in AL bone marrow mononuclear cells of 27 children were detected by modified telomeric repeat PCR and reverse transcriptase polymerase chain reaction. The results were compared with those of 9 normal bone marrow mononuclear cells p15 gene expression were compared. Results At the initial diagnosis, the telomerase activity of bone marrow cells in AL children (34.5 ± 37.0%) was significantly higher than that of the control group (2.4 ± 2.2%) (P <0.001) The gene expression level (9.8 ± 16.2%) was significantly lower than that of the control group (45.8 ± 16.9%) (P <0.001). AL children with bone marrow cell telomerase activity and p15 gene expression was not related (r = 0.01304, P> 0.05). Conclusion The decrease of telomerase activation and p15 gene expression is associated with the development of acute myeloid leukemia, but both may play a role in leukemia through different mechanisms.