论文部分内容阅读
采用室内培养法研究了葡萄糖与氮肥添加下棕壤中三种微生物来源氨基糖含量对恒湿处理和干湿交替处理的响应,同时利用氨基葡萄糖/胞壁酸比值探讨了土壤微生物群落结构的相对变化。结果表明,与恒湿处理相比,当土壤中底物充足时,干湿交替处理有利于土壤中胞壁酸﹑氨基葡萄糖和氨基半乳糖含量的积累,而当底物缺乏时3种氨基糖又发生不同程度的分解。总体来看,土壤中胞壁酸的转化更容易受到干湿交替处理的影响,而对氨基半乳糖影响很小,表明干湿交替改变了以氨基糖为代表的土壤氮素的微生物转化过程。此外,与恒湿处理相比,干湿交替改变了土壤中微生物的群落结构,前期刺激了细菌的生长,抑制了真菌的活性,随着干湿频率次数的增多,又抑制了细菌的生长,刺激了真菌的活性。到培养结束时,干湿交替处理下微生物的群落结构与恒湿处理相平衡。
The indoor culture method was used to study the response of amino sugar content of three kinds of microorganisms in brown soil to the treatment of constant humidity and wetting and drying alternately under the addition of glucose and nitrogen fertilizers. Meanwhile, the relationship between soil microbial community structure and glucosamine / Variety. The results showed that compared with the constant-humidity treatment, when the substrate in the soil was adequate, the alternate treatment of wetting and drying was conducive to the accumulation of the content of muramic acid, glucosamine and galactosamine in the soil, while when the substrate was deficient, three kinds of amino sugar Another occurred in different degrees of decomposition. Overall, the conversion of mural acid in soil was more easily affected by the alternation of wet and dry, but had little effect on the content of amino galactose, which indicated that the dry-wet alternation changed the microbial transformation of soil nitrogen represented by amino sugar. In addition, compared with the constant-humidity treatment, the wet-dry alternation changed the community structure of microorganisms in the soil, stimulated the growth of the bacteria in the early stage and inhibited the activity of the fungi. With the increase of the number of wet and dry cycles, Stimulates fungal activity. By the end of incubation, the community structure of microorganisms under alternating wet and dry treatments is in balance with the constant-humidity treatment.