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目的:观察大承气颗粒抗小鼠急性细菌性腹膜炎的作用。方法:采用给小鼠腹腔注射腹膜炎形成液的方法建立小鼠急性细菌性腹膜炎模型,比色法测定血清NO含量,放射免疫分析法测定血清TNF-α、IL-6及IL-1β的含量,并观察小肠及肝组织的形态学变化。结果:模型组小鼠血清NO、TNF-α、IL-6及IL-1β的含量明显增加,小肠及肝脏组织损伤加重;大承气颗粒给药组血清NO、TNF-α、IL-6及IL-1β的含量有不同程度的降低,小肠及肝组织的病理损伤减轻。结论:大承气颗粒能够通过减少炎症介质和细胞因子的生成,减轻对腹膜炎小鼠的损伤。
Objective: To observe the effects of Dachengqi Granules on acute bacterial peritonitis in mice. METHODS: The mouse model of acute bacterial peritonitis was established by intraperitoneal injection of peritonitis forming solution. Serum NO was measured by colorimetric method, and serum TNF-α, IL-6 and IL-1β levels were determined by radioimmunoassay. Observe the morphological changes in the small intestine and liver tissue. RESULTS: The levels of NO, TNF-α, IL-6 and IL-1β in the mice of the model group were significantly increased, and the damage of the small intestine and liver tissues was aggravated; the serum levels of NO, TNF-α and IL-6 in the Dachengqi Granule group were significantly increased. The content of IL-1β was decreased to some extent, and the pathological lesions of the small intestine and liver tissues were alleviated. Conclusion: Dachengqi Granules can reduce the damage to peritonitis mice by reducing the production of inflammatory mediators and cytokines.