我们对液氮罐内气相温层进行了分析研究,并设计了一种简易器械降温,并进行了胎脑细胞冻存的实验研究。现报告如下: 材料与方法 首先将液氮充入液氮罐(成都液氮生物容器厂生产YDS-10)内40mm深,(液氮在液氮罐内的液相深度),加盖静置24h,去盖换上自制的钢质连接体(见附图),固定升降 We have carried on the analysis research to the gaseous phase temperature layer in the liquid nitrogen tank, and has designed a kind of simple instrument to cool down, and carried on the embryonic brain cell frozen storage experiment research. The report is as follows: Materials and methods Firstly, liquid nitrogen is filled into a liquid nitrogen tank (YDS-10 produced in Chengdu Liquid Nitrogen Bioproduction Plant) within 40 mm depth (the liquid phase depth of the liquid nitrogen in the liquid nitrogen tank) 24h, to replace the cover made of steel connectors (see photo), fixed lift