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目的研究压力超负荷致心肌肥厚模型大鼠心脏结构的变化特点及小檗碱对此的干预作用。方法采用腹主动脉结扎法制备压力超负荷型心肌肥厚大鼠模型,造模后2周开始给药,连续10周。以左心室前壁厚度、左心宣腔横切面积、组织羟脯氨酸含量以及心肌间质胶原容积分数(ICVF)等作为心肌肥大和重塑的指标,观察药物的作用。结果结扎腹主动脉12周后,模型组动物心室前壁厚度、心室腔横切面积及左心室壁横切面积均较假手术组动物增加,并显示同心性肥大的特点。且左心室组织羟脯氨酸含量及ICVF均比假手术对照组明显增加,表明心肌间质胶原沉积是压力超负荷致心肌肥厚和重塑的特征之一。小檗碱5、10、20 mg/kg和阳性对照药物卡托普利45 mg/kg连续给药10周对上述变化有一定改善作用。结论小檗碱可能通过抑制心肌间质纤维化而改善心室重构。
OBJECTIVE: To study the changes of cardiac structure in rats with myocardial hypertrophy induced by pressure overload and the intervention effects of berberine. Methods The rat model of pressure overload cardiac hypertrophy was prepared by abdominal aortic ligation. The administration was started 2 weeks after the model was established for 10 consecutive weeks. The left ventricular anterior wall thickness, the area of the left ventricular cavity, tissue hydroxyproline content, and myocardial interstitial collagen volume fraction (ICVF) were used as indicators of myocardial hypertrophy and remodeling, and the effects of the drug were observed. Results After ligation of the abdominal aorta for 12 weeks, the thickness of the ventricle anterior chamber wall, the area of the ventricle cavities, and the area of the left ventricle wall were increased in the model group compared with the sham-operated group, and the characteristics of concentric hypertrophy were shown. The content of hydroxyproline and ICVF in left ventricle tissue were significantly higher than those in sham-operated control group, indicating that collagen deposition in myocardial interstitium is one of the characteristics of cardiac hypertrophy and remodeling caused by pressure overload. Berberine 5, 10, 20 mg/kg and the positive control drug captopril 45 mg/kg were administered continuously for 10 weeks to improve the above changes. Conclusion Berberine may improve ventricular remodeling by inhibiting myocardial interstitial fibrosis.