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目的结合病毒分离技术,运用RT-PCR法对腮腺炎病毒的核酸进行检测,为快速确诊提供参考。方法采用中国疾病预防控制中心提供的RT-PCR法,结合病原学检测技术对2010-2013年已检测出的腮腺炎病毒进行复检。结果对近4年分离到的18份腮腺炎病毒分离液进行检测,病毒核酸结果均为阳性;应用病毒分离技术检测,有15份为阳性,经中国疾病预防控制中心鉴定为F基因型。结论该方法比病毒分离方法敏感性高,所需时间短,仅需要5~6个小时即可得出结果,为可靠的实验室诊断方法。
Objective To detect the mumps virus nucleic acid by RT-PCR in combination with virus isolation technology and provide a reference for rapid diagnosis. Methods The mumps virus detected in 2010-2013 was retested by RT-PCR method provided by China Center for Disease Control and Prevention (CDC) combined with etiological detection technology. Results A total of 18 mumps virus isolates were isolated from the past 4 years. All of the virus nucleic acid results were positive. Fifteen samples were positive by virus isolation and identified as F genotypes by the Chinese Center for Disease Control and Prevention. Conclusion The method is more sensitive than the virus isolation method, the time required is short, and only takes 5 ~ 6 hours to get the result, which is a reliable laboratory diagnostic method.