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【目的】探讨喘息性疾病患儿外周血T淋巴细胞亚群的表达及意义。【方法】应用流式细胞仪检测哮喘急性发作组、喘息性肺炎组及正常对照组儿童外周血T细胞亚群表达水平。【结果】哮喘急性发作组和喘息性肺炎组CD3+淋巴细胞百分比均较正常对照组明显降低(P<0.01),且二者降低水平一致(P>0.05)。哮喘急性发作组CD4+淋巴细胞百分比较正常对照组和喘息性肺炎组均明显增高(P<0.05),且喘息性肺炎组CD4+淋巴细胞百分比与对照组比较差异也有统计学意义(P<0.05)。哮喘急性发作组及喘息性肺炎组CD8+淋巴细胞百分比与正常对照组比较明显降低(P<0.05),而哮喘急性发作组及喘息性肺炎组之间差异无统计学意义(P>0.05)。哮喘急性发作组和普通喘息组CD3-CD19+和CD19+CD23+较正常对照组明显升高,差异有统计学意义(P<0.05),但这些指标在哮喘急性发作组和普通喘息组之间差异均无统计学意义(P>0.05)。【结论】细胞免疫功能紊乱参与儿童喘息性疾病的发病。
【Objective】 To investigate the expression and significance of T lymphocyte subsets in children with asthmatic disease. 【Methods】 The expression of T lymphocyte subsets in peripheral blood of children with acute asthma, asthmatic pneumonia and normal controls were detected by flow cytometry. 【Results】 The percentages of CD3 + lymphocytes in acute asthma group and asthmatic pneumonia group were significantly lower than those in normal control group (P <0.01), and the levels of CD3 + lymphocytes were the same (P> 0.05). The percentages of CD4 + lymphocytes in acute asthma group were significantly higher than those in normal control group and asthmatic pneumonia group (P <0.05). The percentage of CD4 + lymphocytes in asthmatic pneumonia group was also significantly different from that in control group (P <0.05). The percentages of CD8 + lymphocytes in acute exacerbation group and asthmatic pneumonia group were significantly lower than those in normal control group (P <0.05), but there was no significant difference between acute asthma group and asthmatic pneumonia group (P> 0.05). The levels of CD3-CD19 + and CD19 + CD23 + in asthmatic acute attack group and normal wheezing group were significantly higher than those in normal control group (P <0.05), but these indices were significantly different between acute exacerbation group and general asthmatic group No statistical significance (P> 0.05). 【Conclusions】 Cellular immune dysfunction is involved in the development of asthmatic children.