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为研究紫草萘醌类化合物的细胞毒性作用机制 ,从新疆软紫草根中分离出四种紫草萘醌类化合物 β ,β 二甲基丙烯酰阿卡宁 (1) ,乙酰阿卡宁 (2 ) ,β 乙酰氧基异戊酰阿卡宁 (3)和阿卡宁 (4)。研究了四种天然紫草萘醌类化合物对乳酸脱氢酶和乙醇脱氢酶的共价修饰作用。酶活力测定结果表明 ,这四种紫草萘醌类化合物对两种脱氢酶都具有不同程度的抑制作用 ;酶分子中游离氨基和巯基修饰率的测定结果表明 ,紫草萘醌类化合物对两种酶的抑制作用主要是通过与酶分子中的巯基共价结合产生的。
In order to study the cytotoxic mechanism of naphthaquinone compounds, four kinds of purpura naphthoquinones β, β dimethylacryloyl akanin (1) and acetyl acamin were isolated from the root 2), β-Acetyl Isovaleroyl Akanine (3) and Akanine (4). The covalent modification of lactate dehydrogenase and alcohol dehydrogenase by four natural comfrey naphthoquinones was studied. The results of enzyme activity assay showed that the four comfreyins had different degrees of inhibition on the two dehydrogenases; the results of the determination of the free amino and thiol modification rates in the enzyme molecules showed that the The inhibitory effect of the two enzymes is mainly produced by the covalent binding of sulfhydryl groups in the enzyme molecule.