四川农业大学颜其贵等,对草鱼细菌性败血症疫苗进行了研究。其方法是,取嗜水气单胞菌各菌株接种于10ml马丁肉汤中,在37℃条件下培养24小时,同时抽取菌液用生物计数法作细菌计数。将上述菌液按 Yan Qigui Sichuan Agricultural University, grass carp bacterial septicemia vaccine research. The method comprises the following steps: taking each strain of Aeromonas hydrophila inoculated into 10 ml of martin broth and cultivating under the condition of 37 DEG C for 24 hours; simultaneously taking the bacterial liquid for counting the bacteria by the biological counting method. The above bacteria press