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本文报告鬼臼毒素的半合成化合物鬼臼酰肼哌啶腙氮氧自由基(GP1)对体外培养人食管癌细胞增殖的抑制作用。用0.05,0.2和1μg/ml的GP1处理细胞72h,平均抑制率分别为12.3％42.5％和60.3％,1μg/ml以上时,抑制率几乎不再增加。GP1抑制细胞增殖的作用与处理时间有关,其对细胞克隆形成率也有明显抑制作用。而在0.2—20μg/ml范围内细胞相对增殖存活率无明显差别。用0.2,1,5μg/ml处理细胞24h或48h检验有丝分裂指数,结果表明GP1对细胞有丝分裂指数均有升高作用。本文还对GP1抑制细胞增殖和升高细胞有丝分裂指数的关系进行了讨论。
This article reports the inhibition of the proliferation of human esophageal cancer cells in vitro by a semi-synthetic compound podophyllotoxin, podophylloyl piperidine nitroxide (GP1). When the cells were treated with 0.05, 0.2, and 1 μg/ml of GP1 for 72 h, the average inhibition rates were 12.3%, 42.5%, and 60.3%, respectively. Above 1 μg/ml, the inhibition rate was almost no longer increased. The effect of GP1 on cell proliferation was related to the treatment time, and it also had a significant inhibitory effect on the cell colony formation rate. There was no significant difference in the relative proliferation and survival rate of cells in the range of 0.2-20 μg/ml. The mitotic index was examined by treating cells at 0.2, 1, and 5 μg/ml for 24 h or 48 h. The results showed that GP1 had an elevated effect on the cell mitotic index. This article also discusses the relationship between GP1 inhibiting cell proliferation and increasing cell mitotic index.