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本文研究了辐射防护剂对照后家兔血清中总硷性磷酸酶(AP)及其骨、肠同功酶活性变化的影响。实验用体重3.5～3.8公斤的雌性家兔,经~(60)℃γ-线全身照射800伦(不超过LD~(50)/30的剂量),剂量率为140伦/分,辐射防护剂用胱胺或5-甲氧色胺;胱胺盐酸盐100毫克/公斤(按硷基计)皮下注射或由耳静脉慢慢输入,5-甲氧色胺盐酸盐30毫克/公斤(按硷基计)腹腔注射,曾于照前15至20分钟给药,对照动物给以相应体积的生理盐水,每组8只,反复由耳静脉取血。血清中AP活性用Stepan等人的热灭活抑制法测定,以对-硝基苯磷酸酯作底物,用分光光度计在405nm处测定释放的
In this paper, the effects of radioprotectants on the changes of total alkaline phosphatase (AP) and the activity of bone and intestine in rabbit serum were studied. Female rabbits weighing 3.5-3.8 kg were immunized with γ-rays at ~ (60) ° C for a total of 800 cycles (up to LD 50/30) at a dose rate of 140 Lun / min. Radioprotective agents With cystamine or 5-methoxytryptamine; cystamine hydrochloride 100 mg / kg (by base) injected subcutaneously or slowly from the ear veins 5-methoxytryptamine hydrochloride 30 mg / kg ( According to the base) intraperitoneal injection, had given 15 to 20 minutes before taking the control animals to the corresponding volume of saline, each group of 8, repeated by the ear vein blood. AP activity in serum was determined by Stepan et al. Heat inactivation inhibition assay using p-nitrophenyl phosphate as a substrate and the release of