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目的:选择经试验验证的miR-21靶基因并分析其相关生物信息学过程,为深入研究miR-21在食管癌发生、发展过程中的分子机制提供了理论依据。方法:通过TarBase检索经实验证实的miR-21靶基因及靶基因验证的具体实验方法、靶向位点、调控方式、文献出处等注释信息,采用证实的基因簇,采用GO(GeneOntology)基因注释及KEGG基因信号通路富集的方法进行生物信息学分析。结果:(1)经检索目前经实验证实的miR-21靶基因数量为525个,其靶基因与多种疾病相关尤其是肿瘤。(2)miR-21的靶基因主要涉及调控核糖体、细胞骨架、基因转录、过渡金属离子结合、DNA结合、核苷酸结合、锌离子结合等生物过程,参与癌症信号通路、MAPK、信报因子受体互动、细胞周期、p53、分子粘附等信号通路。结论:miR-21的靶基因可能通过调控多个信号通路参与食管癌的发病机制,且这些信号同理可能以癌症相关信号通路为主。
Objective: To select the validated miR-21 target gene and analyze the related bioinformatics process, which provides a theoretical basis for further study on the molecular mechanism of miR-21 in the occurrence and development of esophageal cancer. Methods: Tarxpression of Targeted Genes (ORFs) was verified by TarBase. The experimental data were validated by real-time RT-PCR. And KEGG gene signal pathway enrichment method for bioinformatics analysis. Results: (1) At present, 525 miR-21 target genes have been confirmed by experiments, and their target genes are associated with various diseases, especially tumors. (2) miR-21 target genes mainly involved in the regulation of biological processes such as ribosomes, cytoskeleton, gene transcription, transition metal ion binding, DNA binding, nucleotide binding, zinc ion binding, etc., involved in cancer signal pathway, MAPK, Factor receptor interaction, cell cycle, p53, molecular adhesion and other signaling pathways. Conclusion: The target gene of miR-21 may be involved in the pathogenesis of esophageal cancer by regulating multiple signal pathways, and these signals may be emphatically related to cancer-related signaling pathways.