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目的探讨轻、重度子痫前期患者和正常产妇胎盘组织中TNF-α和MSTN的表达差异与子痫前期发病程度的关系。方法选择足月健康产妇40例为正常妊娠组、确诊轻度子痫前期产妇40例作为实验A组、重度子痫前期产妇40例为实验B组。免疫组化SP法检测TNF-α和MSTN在胎盘组织中的定位,反转录-半定量PCR(RT-PCR)及蛋白印迹法(Western blot)检测胎盘组织中TNF-α和MSTN mRNA和蛋白的表达差异。结果各组胎盘组织中均有TNF-α和MSTN表达,位置基本相同。TNF-α和MSTN mRNA和蛋白的表达水平在A组和B组明显高于对照组,差异有统计学意义(P<0.01)。B组显著高于A组,差异有统计学意义(P<0.01)。结论子痫前期患者胎盘组织中TNF-α和MSTN的分子生物学的变化,可能是子痫前期病情加重的机制之一。
Objective To investigate the relationship between the expression of TNF-α and MSTN in the mild and severe preeclampsia and normal maternal placenta and the incidence of preeclampsia. Methods Forty healthy full-term pregnant women were selected as normal pregnancy group. Forty pregnant women with mild preeclampsia as experimental group A and 40 as severe pregnant women with preeclampsia were selected as experimental group B. The localization of TNF-α and MSTN in placenta was detected by immunohistochemistry SP method. The mRNA and protein of TNF-α and MSTN in placenta were detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting Differences in expression. Results The expression of TNF-α and MSTN in placenta of all groups were basically the same. The mRNA and protein expressions of TNF-α and MSTN in group A and group B were significantly higher than those in control group (P <0.01). B group was significantly higher than A group, the difference was statistically significant (P <0.01). Conclusions The molecular biological changes of TNF-α and MSTN in placenta of preeclampsia may be one of the mechanisms of aggravating preeclampsia.