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目的了解洁净手术室术间空气细菌密度影响因素,探讨相应的护理对策。方法对5间千级、万级和十万级手术室的45台手术进行动态条件下空气细菌检测,检测方法采用自然沉降法,结果计算后用CFU/m3表示。结果所有手术间的术前空气含菌量均为0～23 CFU/m3,随着手术时间的延续空气细菌数逐渐增加,不同手术持续时间的术后检测:<1 h组为202～778 CFU/m3,1～2 h组为582～1332 CFU/m3,2～3 h组为672～1993 CFU/m3,>3 h组为713～2108 CFU/m3;不同手术人员数的术后检测:≤4人组为202～471 CFU/m3,5～8人组为355～901 CFU/m3,9～12人组为614～1443 CFU/m3,≥13人组为878～2108 CFU/m3;不同术中开门次数的术后检测:≤6次组为202～328 CFU/m3,7～11次组为562～889 CFU/m3,12～16次组为673～1870CFU/m3,≥17次组为882～2108 CFU/m3。结论影响术间空气细菌含量与手术时间长短,室内人员数,以及术间开门的次数有关,为了减缓手术过程空气细菌密度增大趋势,必须加强术间动态条件下的检测和一系列有效的预防措施。
Objective To understand the influencing factors of air bacterial density in clean operating room and to discuss the corresponding nursing measures. Methods Forty-five surgeries in five thousand, ten thousand and one hundred thousand operation rooms were tested for airborne bacteria under the dynamic conditions. The natural sedimentation method was used for the detection and the results were expressed as CFU / m3. Results All the preoperative airborne bacteria were 0 ~ 23 CFU / m3. The number of airborne bacteria increased with the duration of operation. The postoperative detection of different operative durations was 202 ~ 778 CFU / m3 for 1 ~ 2 h, 582 ~ 1332 CFU / m3 for 1 ~ 2 h, 672 ~ 1993 CFU / m3 for 2 ~ 3 h and 713 ~ 2108 CFU / m3 for 3 h. Postoperative detection of different operatives: The patients in the range of ≤4 were 202 ~ 471 CFU / m3, those in the range of 5 ~ 8 were 355 ~ 901 CFU / m3, those in the range of 9 ~ 12 were 614 ~ 1443 CFU / m3, those in ≥13 were 878 ~ 2108 CFU / The number of open surgery in different postoperative detection: ≤ 6 times the group of 202 ~ 328 CFU / m3, 7 ~ 11 times the group of 562 ~ 889 CFU / m3, 12 ~ 16 times the 673 ~ 1870CFU / m3, ≥ 17 times Group for the 882 ~ 2108 CFU / m3. Conclusion The airborne bacteria in the air is related to the length of operation, the number of people in the room and the number of door opening during operation. In order to reduce the trend of increasing airborne bacteria density during operation, it is necessary to strengthen the detection under dynamic conditions and a series of effective preventive measures Measures.