论文部分内容阅读
目的:建立痹康颗粒的质量标准。方法:采用薄层色谱法对痹康颗粒中当归、丁香进行定性研究,并采用高效液相色谱法对方中补骨脂素、异补骨脂素进行含量测定研究。色谱柱:AgilentXDB-C18柱(4.6mm 150mm,5μm);流动相:甲醇-水(45:55);检测波长:246nm;流速:1.0ml/分钟;柱温:25℃。结果:薄层色谱斑点清晰,阴性无干扰;补骨脂素在浓度范围8.64～43.20mg/ml内呈良好的线性关系,r=0.9998,平均加样回收率为97.33%,RSD=1.08%(n=6);异补骨脂素在浓度范围8.92～44.60mg/ml内呈良好的线性关系,r=0.9997,平均加样回收率为98.38%,RSD=0.92%(n=6)。结论:该方法简便、准确、可靠,可以作为痹康颗粒的质量控制方法。
Objective: To establish the quality standard of BiKang granules. Methods: The contents of Angelica and Clove in Bikang Granules were qualitatively studied by TLC, and the contents of psoralen and isopsoralen in the prescription were determined by HPLC. Column: AgilentXDB-C18 column (4.6mm 150mm, 5μm); mobile phase: methanol-water (45:55); detection wavelength: 246nm; flow rate: 1.0ml / min; Results: The TLC spots were clear and negative without interference. The concentration of psoralen showed a good linearity in the concentration range of 8.64 ~ 43.20mg / ml, r = 0.9998, the average recovery was 97.33% and the RSD was 1.08% n = 6). Isopsoralen showed a good linearity in the concentration range of 8.92 ~ 44.60mg / ml with r = 0.9997. The average recovery was 98.38% with RSD = 0.92% (n = 6). Conclusion: The method is simple, accurate and reliable and can be used as a quality control method for Bikang granules.