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目的:确定HPD300大孔吸附树脂吸附纯化石香薷总黄酮的工艺条件。方法:采用静态吸附和动态吸附分离法,确定石香薷总黄酮的纯化工艺条件。结果:HPD300大孔树脂对石香薷总黄酮有良好的吸附分离性能,其动态吸附分离工艺条件为:石香薷总黄酮上样液(pH=3.0)浓度为5.5 mg/mL,最大吸附量为41.58 mg/g,吸附流速为2.0 mL/min,以8倍柱体积60%乙醇(pH=8.0)洗脱,树脂可重复使用4次。此工艺下石香薷总黄酮的回收率达89.12%,纯度为90.77%。结论:采用HPD300大孔树脂吸附分离石香薷总黄酮简便有效,回收率高。
Objective: To determine the adsorption conditions of HPD300 macroporous resin for purification of total flavonoids from Perilla frutescens. Methods: The static adsorption and dynamic adsorption separation methods were used to determine the purification conditions of total flavonoids of Perilla frutescens. Results: HPD300 macroporous resin had good adsorption and separation performance on total flavonoids of Perilla frutescens. The dynamic adsorption and separation conditions were as follows: the concentration of total flavonoids (pH = 3.0) of Perilla frutescentis was 5.5 mg / mL and the maximum adsorption capacity was 41.58 mg / g, the adsorption flow rate was 2.0 mL / min and the column was eluted with 8 column volumes of 60% ethanol (pH = 8.0). The resin could be reused for 4 times. Under this process, the recovery rate of total flavonoids of Perilla frutescens was 89.12% and the purity was 90.77%. Conclusion: Adsorption and separation of total flavonoids from Perilla frutescens leaves by HPD300 macroporous resin is simple and effective.