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目的:观察芪茵颗粒对实验性非酒精性脂肪肝(NAFL)大鼠生化指标和肝脏组织病理的影响,分析芪茵颗粒对非酒精性脂肪肝(NAFL)的干预效果。方法:60只Wistar大鼠雌雄各半,随机分为六组:空白组、模型对照组、易善复组、芪茵颗粒低、中、高剂量,每组10只。空白对照予以常规饲养,其余五组给予高脂乳剂持续喂养8周,随机抽取大鼠6只,确定造模成功。除空白组予以标准饲料喂养,并以同等剂量的蒸馏水每日灌胃,其余五组均继续以高脂乳剂15mL/kg灌胃,并分别给予芪茵颗粒低、中、高剂量、易善复灌胃,连续12周后处死大鼠,测定血清甘油三脂(TG)、胆固醇(CHOL),高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、丙氨酸氨基转移酶(ALT)、肝重、肝指数;HE染色后光镜下观察肝脏组织病理学改变。结果:芪茵颗粒可降低NAFL大鼠血清总胆固醇(CHOL)、低密度脂蛋白(LDL-C)含量,肝重、肝指数(P<0.05),改善脂肪肝大鼠肝脏脂肪变(P<0.01)。结论:芪茵颗粒可降低NAFL大鼠血清CHOL、TG、LDL-C的含量,升高HDL-C含量,改善肝脏脂肪变,从而达到治疗NAFL的目的。
OBJECTIVE: To observe the effect of Quyin Granules on the biochemical indexes and hepatic histopathology of experimental non-alcoholic fatty liver (NAFL) rats, and to analyze the intervention effect of Quyin Granules on non-alcoholic fatty liver (NAFL). METHODS: Sixty Wistar male and female rats were divided into six groups: blank group, model control group, Yi Shan Fu group, and low dose, medium dose and high dose of Cao Yin granules. Each group contained 10 rats. The blank control group was fed routinely. The remaining five groups were given high-fat emulsion for 8 weeks. Six rats were randomly selected and the model establishment was confirmed. Except for the blank group, they were fed standard diets and fed the same dose of distilled water daily. The remaining five groups continued to be fed with high-fat emulsion 15 mL/kg, and were given low, medium, and high doses of BBG, respectively. After intragastric administration for 12 weeks, rats were sacrificed and serum triglyceride (TG), cholesterol (CHOL), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), alanine were measured. Aminotransferase (ALT), liver weight, and liver index; Histopathological changes of the liver were observed under light microscope after HE staining. Results: Astragalus granules reduced serum total cholesterol (CHOL), low density lipoprotein (LDL-C), liver weight and liver index (P<0.05), and improved hepatic steatosis in rats with fatty liver (P<0.05). 0.01). Conclusion: The ginseng granules can reduce the content of CHOL, TG, and LDL-C in serum of NAFL rats, increase the content of HDL-C, and improve the hepatic steatosis, so as to achieve the purpose of treating NAFL.