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Objective: To observe the effects of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor (PDGF) in vascular smooth muscle cells (VSMCs) of rats. Methods: The fifth to tenth passage culture of VSMCs was used, tripterine and 20% fetal calf serum added into the medium of cultured VSMCs at concentration of 0. 1 mg/L, 0. 2 mg/L and 0. 3 mg/L after serum-free cultivation for 24 hours.The general RNA was isolated from VSMCs at 6 and 12 hours after the drug addition for detection of oncogene c-myc and PDGF mRNA respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. Results: Tripterine inhibited the expression of PDGF mRNA of VSMCs, and decreased expression of oncogene cmyc mRNA in a dose-dependent manner, either vs. control. Conclusion: Tripterine can inhibit expression of oncogene c-myc and PDGF-A mRNA in VSMCs, therefore it would inhibit overproliferation of VSMCs.
Methods: The fifth to tenth passage culture of VSMCs was used, tripterine (VSMCs) of rats. Methods: The fifth to tenth passage culture of tripterine on mRNA expression of oncogene c-myc and platelet-derived growth factor (PDGF) and 20% fetal calf serum added into the medium of cultured VSMCs at a concentration of 0.1 mg / L, 0.2 mg / L and 0.3 mg / L after serum-free cultivation for 24 hours.The general RNA was isolated from VSMCs at 6 and 12 hours after the drug addition for detection of oncogene c-myc and PDGF mRNA respectively by dot blot hybridization. The cDNA probes were labeled by digoxin. Results: Tripterine inhibited the expression of PDGF mRNA of VSMCs, and decreased expression of oncogene cmyc mRNA in a dose-dependent manner, either vs. control. Conclusion: Tripterine can inhibit expression of oncogene c-myc and PDGF-A mRNA in VSMCs, therefore it would inhibit overproliferation of VSMCs.