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目的探讨骨桥蛋白(OPN)作为大肠癌肿瘤标志物检测的临床意义。方法 2007年1月至2013年12月收治212例大肠癌患者,应用酶联免疫吸附试验(ELISA)检测患者手术治疗前后血清0PN的质量浓度,用免疫组织化学法测定肿瘤组织OPN的表达。对比血清OPN在手术前后的变化,并与对照组(正常健康志愿者)60例比较;分析血清OPN与大肠癌组织OPN的表达、临床特征和病理特征的相关性。结果血清OPN在大肠癌中检测的敏感性和特异性分别为70.3%(149/212)和83.5%(177/212)。大肠癌血清OPN水平依肿瘤组织OPN表达阳性率由低到高的顺序递升,差异有统计学意义(P<0.05);并与临床分期、脉管内癌栓、淋巴结和器官转移相关(P<0.05或P<0.01),特别与肝转移密切相关(P<0.01),与肿瘤大小及分化程度无相关性(P均>0.05)。治疗前后OPN的质量浓度差异有统计学意义(P<0.01)。结论 OPN是一种较理想的大肠癌肿瘤标志物。
Objective To investigate the clinical significance of osteopontin (OPN) as a tumor marker in colorectal cancer. Methods From January 2007 to December 2013, 212 patients with colorectal cancer were enrolled. Serum 0PN levels were measured by enzyme-linked immunosorbent assay (ELISA) before and after operation. The expression of OPN was detected by immunohistochemistry. The changes of serum OPN before and after surgery were compared with those in the control group (normal healthy volunteers) 60 cases. The correlation between OPN expression and clinical features and pathological features of OPN in colorectal cancer tissues was analyzed. Results The sensitivity and specificity of serum OPN in colorectal cancer were 70.3% (149/212) and 83.5% (177/212), respectively. The level of OPN in colorectal cancer increased gradually from low to high in OPN group, the difference was statistically significant (P <0.05), and correlated with clinical stage, intravascular thrombosis, lymph node and organ metastasis (P <0.05 Or P <0.01), especially closely related to liver metastasis (P <0.01), but not with tumor size and differentiation (P> 0.05). Before and after treatment OPN quality concentration difference was statistically significant (P <0.01). Conclusion OPN is a more ideal tumor marker of colorectal cancer.