对双生病毒编码基因的变异进行了密码子用法分析,推测双生病毒基因的表达水平普遍不高,而外壳蛋白基因的表达水平相对较高,它们在密码子第3位上偏好使用A或T。双生病毒的密码子用法主要受到突变偏好和翻译选择等因素的影响。双生病毒的密码子使用具有基因特异性和一定程度的寄主特异性。基于相对同义密码子使用频率的聚类分析可以很好反映侵染单子叶植物和双子叶植物双生病毒之间的差异,也在一定程度上反映出双生病毒之间的亲缘关系。 The analysis of the codon usage of the gene encoding the bipyridovirus led to the generally low level of expression of the geminiviruses and the relatively high level of expression of the coat protein genes. They preferred A or T at codon 3. The codon usage of geminiviruses is mainly influenced by factors such as mutation preference and translation choice. Gemini virus codon usage is gene specific and to some extent host specific. Clustering analysis based on the relative synonymous frequency of codons can well reflect the difference between monocotyledonous and dicotyledonous infested viruses and to a certain extent reflect the relationship between the geminiviruses.