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目的:对重庆市2007年分离的人群沙门菌菌株进行耐药性和PFGE分子分型研究。方法:全自动细菌生化鉴定仪(VITEK32)和血清学鉴定分离的人群沙门菌,利用K-B法对分离菌株进行药敏检测,并采用PFGE进行分子分型。结果:2007年共分离鉴定79株12种沙门菌(11种血清型和1组B群),全部菌株对至少一种抗菌素耐药,75%(9种)对3种以上的抗菌素耐药,16.67%(2种)对6种抗菌素耐药,尤其是里定沙门菌、婴儿沙门菌及汤卜逊沙门菌耐药严重。沙门菌被分成9种PFGE型14种亚型。结论:重庆市2007年人群沙门菌分离株没有明显的优势流行株,同一事件中的沙门菌PFGE分型相同,分离株耐药情况严重,分离菌株的耐药谱和PFGE型也没有明显的联系。
Objective: To study the drug resistance and PFGE molecular typing of Salmonella isolates from Chongqing population in 2007. Methods: Salmonella were isolated from patients with VITEK32 and serodiagnosis. K-B method was used to detect the susceptibility of the isolated strains and PFGE was used for molecular typing. Results: In 2007, 79 strains of 12 Salmonella species (11 serotypes and 1 group B) were isolated and identified. All strains were resistant to at least one antibiotic, 75% (9 species) were resistant to more than 3 antibiotics, 16.67% (2 species) were resistant to 6 antibiotics, especially Salmonella typhimurium, Salmonella paratyphi and Salmonella tybus. Salmonella is divided into nine subtypes of nine PFGE types. Conclusion: There was no obvious prevalent epidemic strain of Salmonella isolates from Chongqing in 2007, and the same type of Salmonella PFGE in the same incident was found. The drug resistance of the isolated strains was serious. There was also no significant relationship between the drug resistance spectrum and the PFGE type of isolates .