论文部分内容阅读
目的:模仿产伤和破坏大鼠尿道周围组织两种方法建立压力性尿失禁动物模型,并对两种动物模型进行鉴定和比较。方法:雌性成年未生产过的Sprague-Dawley大鼠45只,取5只直接开腹测量尿动力学作为对照组,其余分为A组20只,B组20只。A组行阴道损伤和双侧卵巢切除术,B组行尿道分离术和双侧卵巢切除术。A和B组大鼠各分为4小组,分别称A1-A4和B1-B4,每小组为5只,分别在术后1周,1、2、3月后测量尿动力学指标LPP和ALPP。每组各取2只大鼠行组织学鉴定。结果:与对照值相比A1～A4组LPP值有统计学意义(A1～A3P<0.01,A4P<0.05),ALPP值有统计学意义(A1～A3P<0.01,A4P<0.05),A1～A4组间比较P>0.05,无明显统计学差异。与对照值相比B1～B4组LPP值有统计学意义(B1～B4P<0.05),ALPP值有统计学意义(B1～B4P<0.01),B1～B4组间比较P>0.05,无明显统计学差异。A组和B组组间比较无明显差异(P>0.05)。动物模型尿道组织学上表现为即肌肉成分的萎缩,断裂及神经分布的减少。结论:两种方法均能成功建立SUI动物模型,两种建立方法无明显差异。两种方法建立的模型在3个月内可维持比较良好的稳定性。模拟产伤法具有操作相对简单、风险小、学习曲线短等特点,值得推广。
OBJECTIVE: To establish a stress urinary incontinence animal model by imitating birth injury and destroying the periurethral tissues of rats, and to identify and compare the two animal models. METHODS: Forty-five female Sprague-Dawley rats were used to measure the urodynamics of the untreated females. Totally, 5 females were randomly divided into group A (n = 20) and group B (n = 20). A group of vaginal injury and bilateral ovariectomy, B group urethral isolation and bilateral ovariectomy. Groups A and B were divided into 4 groups, A1-A4 and B1-B4 respectively, and 5 in each group. The urodynamic parameters LPP and ALPP were measured at 1 week, 1, 2 and 3 months after operation . Taken 2 rats from each group for histological identification. Results: Compared with the control group, the LPP values in A1 ~ A4 group were statistically significant (A1 ~ A3P <0.01, A4P <0.05) and ALPP values were statistically significant (A1 ~ A3P <0.01, A4P < Between groups P> 0.05, no significant statistical difference. Compared with the control group, the LPP values of B1 ~ B4 group were statistically significant (B1 ~ B4P <0.05), ALPP value was statistically significant (B1 ~ B4P <0.01), B1 ~ B4 group P> 0.05, Differences There was no significant difference between group A and group B (P> 0.05). Animal models Urethral histology is manifested as muscle atrophy, rupture and reduction of nerve distribution. Conclusion: SUI animal model can be successfully established by both methods. There is no significant difference between the two methods. The model established by the two methods can maintain relatively good stability within 3 months. Simulated birth trauma with relatively simple operation, low risk, learning curve is short, it is worth promoting.