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目的:探讨蝙蝠葛酚性碱(PAMD)对阿尔茨海默病(AD)模型小鼠学习记忆和海马的影响。方法:C57BL/6小鼠随机分成对照组,模型组,吡拉西坦组(620 mg·kg-1),PAMD高、中、低剂量组(30,15,7.5 mg·kg-1)。采用D-半乳糖(180 mg·kg-1)ip和双侧海马微量注射(10μg)β-淀粉样蛋白1-42(Aβ1-42,2 g·L-1)诱导AD模型小鼠。所有小鼠给予等体积药物或生理盐水ig,持续28 d。采用Morris水迷宫测试行为学指标,观察小鼠学习记忆能力及小鼠海马镜下形态结构,采用双抗体夹心法测定脑组织β-淀粉样蛋白(Aβ)和白介素-6(IL-6)水平。结果:与对照组比较,模型组小鼠潜伏期和游泳距离明显增加(P<0.05),Aβ和IL-6水平明显升高(P<0.05)。与模型组比较,PAMD高、中剂量组明显改善AD模型小鼠的学习记忆能力(P<0.05),明显改善海马神经元的细胞形态,明显降低Aβ和IL-6的水平(P<0.05)。结论:PAMD改善AD模型小鼠的学习记忆功能及海马神经元的形态结构,PAMD通过抑制Aβ和IL-6蛋白在AD治疗中发挥重要作用。
Objective: To investigate the effects of PAMD on learning and memory and hippocampus in Alzheimer’s disease (AD) model mice. Methods: C57BL / 6 mice were randomly divided into control group, model group, piracetam group (620 mg · kg -1), PAMD high, medium and low dose groups (30,15,7.5 mg · kg -1). AD model mice were induced with D-galactose (180 mg · kg -1) ip and bilateral hippocampal microinjection (10 μg) of β-amyloid 1-42 (Aβ 1-42, 2 g · L -1). All mice were given an equal volume of drug or saline ig for 28 days. Morris water maze test behavioral indexes were used to observe the ability of learning and memory of mice and the morphology of hippocampus in mice. The levels of β-amyloid protein (Aβ) and interleukin-6 (IL-6) were measured by double antibody sandwich method . Results: Compared with the control group, the latency and swimming distance of model mice increased significantly (P <0.05), while the levels of Aβ and IL-6 increased significantly (P <0.05). Compared with model group, PAMD high and medium dose groups significantly improved the learning and memory abilities of AD model mice (P <0.05), and significantly improved the cell morphology of hippocampal neurons and significantly decreased the levels of Aβ and IL-6 (P <0.05) . Conclusion: PAMD can improve the learning and memory function and the morphological structure of hippocampal neurons in AD model mice. PAMD plays an important role in the treatment of AD by inhibiting Aβ and IL-6 proteins.