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目的：观察维生素 Ｅ（ Ｖ Ｅ）及其联合维生素 Ｃ（ Ｖ Ｃ）对肝细胞抗氧化作用及其增殖的调节作用。方法：采用硫代巴比妥酸法检测肝脏丙二醛（ Ｍ Ｄ Ａ）、流式细胞术检测肝细胞周期。结果：老龄组肝脏 Ｍ Ｄ Ａ 含量明显高于成年对照组，补以 Ｖ Ｅ 或 Ｖ Ｅ＋ Ｖ Ｃ 后 Ｍ Ｄ Ａ 明显下降，尤以 Ｖ Ｅ＋ Ｖ Ｃ 效果显著；与成年对照相比，老龄组肝细胞发生 Ｇ１ 期阻滞，施以上述抗氧化剂处理后， Ｇ０／ Ｇ１、 Ｓ、 Ｇ２＋ Ｍ 各期细胞数均发生逆转性改变。结论： Ｖ Ｅ 具有明显的抗氧化作用，与 Ｖ Ｃ 联用效果更佳，并可改善肝细胞增殖活力。
Objective: To observe the regulation of vitamin E (V E) and its combined vitamin C (V C) on the anti-oxidation and proliferation of hepatocytes. Methods: Liver MDA was detected by thiobarbituric acid method and hepatocyte cycle was detected by flow cytometry. Results: Compared with adult control group, the content of MAD in liver of aged group was significantly higher than that of adult control group. M D A of VE or V E + V C was significantly decreased, especially V E + V C. Compared with adult control group, The G1 arrest occurred in cells. After being treated with the above antioxidants, the number of cells in G0 / G1, S, G2 + M phases changed reversibly. CONCLUSION: V E has obvious anti-oxidation effect, better effect with V C and can improve hepatocyte proliferation activity.