论文部分内容阅读
通过皂苷含量与基因表达量之间相关性分析,获得西洋参中不同组织部位皂苷含量与人参皂苷生物合成相关基因表达之间联系。以四年生红果期西洋参的14个组织部位为材料,利用高效液相法和香草醛-硫酸比色法测定西洋参样本中6种单体皂苷(人参皂苷Rg_1,Re,Rb_1,Rc,Rb_2和Rd),分组皂苷和总皂苷含量;同时,利用实时荧光定量PCR法检测西洋参不同组织部位中7种(SQS,OSC,DS,β-AS,SQE,P450和FPS)人参皂苷生物合成相关基因的表达量。在西洋参中7种人参皂苷生物合成相关酶基因虽然参与人参皂苷合成,但是β-AS和P450基因的表达对单体皂苷,分组皂苷及总皂苷含量影响不显著;FPS,SQS,OSC,DS和SQE这5种基因表达对单体皂苷Re,Rg_1,Rb_1,Rd,分组皂苷PPD和PPT,单体总皂苷TMS和总皂苷TS含量影响显著或者极显著(P<0.05或P<0.01)。西洋参中部分单体皂苷,分组皂苷以及总皂苷的生物合成受着皂苷合成途径多种酶基因相互作用影响,生物合成途径中关键酶基因表达差异决定了西洋参中不同组织部位皂苷含量。因此,本研究通过相关性分析进一步明确了FPS,SQS,OSC,DS和SQE这5种酶基因是控制西洋参中皂苷合成的关键酶,它们通过集群协同表达互作的方式调控西洋参中人参皂苷的生物合成。
Through the correlation analysis between the content of saponin and gene expression, we got the relationship between the content of saponin in different tissues of ginseng and the gene expression of ginsenoside biosynthesis. The 14 tissue fractions of Panax quinquefolius in four-year-old Chinese red ginseng were used as materials. The contents of six ginsenosides (ginsenosides Rg_1, Re, Rb_1, Rc, Rb_2 and Rd ), Grouping saponin and total saponin content. Meanwhile, the expression of ginsenoside biosynthesis genes in seven tissues (SQS, OSC, DS, β-AS, SQE, P450 and FPS) in different tissues of American ginseng was detected by real- the amount. Although the genes involved in the biosynthesis of seven ginsenosides in Panax quinquefolius were involved in the synthesis of ginsenosides, the expression of β-AS and P450 genes had no significant effect on the content of monomeric saponins, group saponins and total saponins. FPS, SQS, OSC, DS and The five gene expressions of SQE had significant or very significant (P <0.05 or P <0.01) effects on the content of monomeric saponins Re, Rg_1, Rb_1, Rd, grouping saponins PPD and PPT, monomeric total saponins TMS and total saponins TS. The biosynthesis of some ginsenosides, ginsenosides and total saponins in American ginseng is influenced by the interaction of many enzymes in the biosynthetic pathway. The differences in the expression of key enzymes in the biosynthetic pathway determine the content of saponins in different tissues of American ginseng. Therefore, we further clarified that FPS, SQS, OSC, DS and SQE are the key enzymes that control the synthesis of saponins in Panax quinquefolium by correlation analysis. They regulate the ginsenosides in Panax quinquefolius Biosynthesis.