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[目的]应用微核实验和彗星电泳实验评价氯化镧亚慢性染毒致小鼠遗传毒性作用。[方法]将100只SPF级ICR小鼠随机分为5组:4个氯化镧染毒组和1个对照组,每组20只,雌雄各半。5个剂量组分别以灌胃的方式给予不同浓度的氯化镧溶液(0、10、20、50和100 mg/kg)。每周染毒6次,连续染毒13周后,取小鼠外周血进行彗星实验及镧含量的测定,取小鼠骨髓观察骨髓细胞微核率。[结果]镧染毒剂量达50 mg/kg时,彗星细胞的尾长及尾部DNA含量与对照组相比,差异有统计学意义(P<0.05),小鼠骨髓细胞微核率与对照组相比,差异有统计学意义(P<0.05)。4个氯化镧染毒组血液中均有不同程度的镧的蓄积,与对照组相比,差异均有统计学意义(P<0.05)。[结论]稀土镧元素可以在血液中蓄积,具有一定的遗传毒性。
[Objective] To evaluate the genotoxic effects of sub-chronic exposure to lanthanum chloride in mice using micronucleus test and comet assay. [Methods] 100 SPF ICR mice were randomly divided into 5 groups: 4 lanthanum chloride poisoning group and 1 control group, each group 20, male and female. The five dose groups were given different concentrations of lanthanum chloride solution (0, 10, 20, 50 and 100 mg / kg) by gavage respectively. 6 times a week, continuous exposure for 13 weeks, take mice peripheral blood comet assay and determination of lanthanum content, bone marrow cells were observed in mice micronuclei. [Result] Compared with the control group, the tail length and the tail DNA content of comet cells were significantly different (P <0.05) at the dose of 50 mg / kg lanthanum. The micronucleus rate of mouse bone marrow cells was significantly lower than that of the control group Compared with the difference was statistically significant (P <0.05). There were different levels of lanthanum accumulation in the four lanthanum chloride poisoning groups compared with the control group, the difference was statistically significant (P <0.05). [Conclusion] The rare earth element lanthanum can accumulate in the blood and has certain genotoxicity.