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目的:在100Hz～100MHz频率范围研究大鼠骨骼肌细胞的电生理特性,为进一步探讨骨骼肌疲劳或力竭的频域电特性研究奠定基础。方法:使用Agilent4294A阻抗分析仪测量大鼠腓肠肌组织的交流阻抗,通过细胞介电谱、Cole-Cole图、介电损失因子△ε″和介电损耗角正切△tgδ的频谱分析,建立了正常大鼠腓肠肌细胞的频域电生理特性参数(介电参数)。结果:(1)大鼠腓肠肌细胞介电响应具有频率依存性关系:介电常数ε随电场频率的增加而降低,电导率κ随频率的增加而上升;(2)大鼠腓肠肌细胞的频域介电参数(κL、κh、εL、εh、△ε″max、△tgδmax、fC1、fC2)在平行方向与垂直方向上存在差异;(3)交流电场对大鼠腓肠肌细胞的作用具有介电弛豫现象,表现为两个中心特征频率:第一特征频率fC1(∥)=fC1(⊥)=1.02kHz和第二特征频率fC2(∥)=59.31kHz,fC2(⊥)=380.04kHz。结论:利用本实验方法可获得骨骼肌细胞频域电生理指标,其中第一特征频率fC1和第二特征频率fC2是频域电特性的特色参数。
OBJECTIVE: To study the electrophysiological characteristics of rat skeletal muscle cells in the frequency range of 100Hz ~ 100MHz, and lay the foundation for the further study on the frequency domain electrical characteristics of skeletal muscle fatigue or exhaustion. METHODS: The AC impedance of rat gastrocnemius muscle was measured by Agilent 4294A impedance analyzer. The spectrum of the dielectric constant, Cole-Cole diagram, dielectric loss factor △ ε “and dielectric loss tangent △ tgδ was established. The frequency-domain electrophysiological parameters (dielectric parameters) of murine gastrocnemius muscle cells were measured.Results: (1) The frequency dependence of dielectric response of rat gastrocnemius cells decreased: the dielectric constant ε decreased with the increase of the electric field frequency, and the conductivity κ (2) The frequency-domain dielectric parameters (κL, κh, εL, εh, △ ε ”max, △ tgδmax, fC1, fC2) of gastrocnemius cells in rats were different in parallel direction and in vertical direction; (3) The effect of AC electric field on rat gastrocnemius cells showed dielectric relaxation phenomenon, which showed two central characteristic frequencies: the first characteristic frequency fC1 (∥) = fC1 (⊥) = 1.02 kHz and the second characteristic frequency fC2 ∥) = 59.31 kHz, fC2 (⊥) = 380.04 kHz. Conclusion: The frequency domain electrophysiological parameters of skeletal muscle cells can be obtained by this method. The first characteristic frequency fC1 and the second characteristic frequency fC2 are the characteristic parameters in frequency domain.