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1案例资料1.1简要案情某日,3名男子在公共场所寻衅滋事逃逸,警察在现场提取到遗留的3枚嫌疑人咀嚼过的槟榔,要求进行DNA分型。1.2 DNA检验样本处理3枚槟榔中2枚较干燥,表面纤维稀疏蓬松,直接剪取槟榔纤维;1枚表面相对潮湿,用无菌脱脂棉在表面进行擦拭。DNA提取及纯化将上述槟榔纤维和擦拭脱脂棉分别置于1.5mL EP管中,加1 000μL纯水清洗,离心,弃上清,保留约100μL液体和沉淀物;加300μL
1 Case Information 1.1 Briefing Case On the other day, three men escaped in public places and the police took three betel nuts chewed by the remaining suspects on site to request DNA typing. 1.2 DNA test sample processing 3 betel nut 2 more dry, surface sparsely fluffy fiber, betel nut fiber directly cut; a surface is relatively humid, wipe with sterile cotton wool on the surface. DNA Extraction and Purification The betel nut fibers and wipe cotton wool were placed in 1.5mL EP tube, add 1000μL of pure water, centrifuged, the supernatant was discarded, leaving about 100μL of liquid and sediment; add 300μL