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利用组织学切片及阿利新蓝-过碘酸雪夫(AB-PAS)组化染色技术对0~50日龄大鳞副泥鳅(Paramisgurnus dabryanus)消化道各段黏液细胞的发育与分布进行显微观察和研究。大鳞副泥鳅黏液细胞分为Ⅰ、Ⅱ、Ⅲ和Ⅳ4种类型。消化道黏液细胞最早出现在4日龄仔鱼的口咽腔和食道。10~15日龄口咽腔和食道黏液细胞数量快速增长,15~20日龄肠道各型黏液细胞数量显著增长,20日龄后消化道黏液细胞分布广泛。随着仔稚鱼发育,消化道各部黏液细胞主要以Ⅲ和Ⅳ型细胞为主。根据大鳞副泥鳅仔稚鱼消化道黏液细胞的发育特点,5~10日龄和15~20日龄为其消化道功能发育的两个敏感时期,20日龄后消化道功能逐渐发育完善。建议加强对5~20日龄仔稚鱼的日常饲养管理以提高苗种成活率。
The development and distribution of mucous cells in the digestive tract of Paramisgurnus dabryanus from 0 to 50 days old were observed microscopically by histological sections and AB-PAS staining. And research. Paramisgirotus molluscum mucus cells are divided into Ⅰ, Ⅱ, Ⅲ and Ⅳ types. Gastrointestinal mucosal cells first appeared in the 4-day-old larvae and esophagus larvae. The number of mucous cells in the oropharynx and esophagus increased rapidly from 10 to 15 days, and the number of mucous cells in each type of intestine increased significantly from 15 to 20 days. Mucosal cells were widely distributed in the digestive tract after 20 days. With the development of larvae, mucosal cells in the digestive tract are mainly composed of type III and type IV cells. According to the development of mucous cells of digestive tract mucus of Paramisgurnus dabryanus, 5 to 10 days old and 15 to 20 days old were two sensitive periods of development of digestive tract function. After 20 days, digestive tract function gradually developed and perfected. Proposed to strengthen the 5 to 20-day-old larvae daily feeding and management to improve seedling survival rate.