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应用反相高效液相色谱法测定格列甲嗪血浆浓度。色谱柱为ＯＤＳＣ１８柱，流动相为乙腈─甲醇─水（４０：２０：４０Ｖ／Ｖ），内含０．１％冰醋酸和０．０８％三乙胺。流速０．５ｍｌ／ｍｉｎ，柱温５０℃，检测波长２７５ｎｍ。线性范围在０．０２～１．００μｇ／ｍｌ（ｒ＝０．９９９９）。检测下限为０．０１μｇ／ｍｌ。平均回收率为９９．０±２．８％，ＲＳＤ＝２．８２％。日内及日间测定相对标准偏差均小于５％。
Determination of glipizide plasma concentration using reversed-phase high performance liquid chromatography. The column was ODSC18 and the mobile phase was acetonitrile-methanol-water (40:20:40 V / V) containing 0.1% glacial acetic acid and 0.08% triethylamine. Flow rate 0.5ml / min, column temperature 50 ℃, detection wavelength 275nm. The linear range was 0.02-1.00 μg / ml (r = 0.9999). The detection limit is 0.01μg / ml. The average recovery was 99.0 ± 2.8% with RSD = 2.82%. The relative standard deviations (RSDs) were less than 5% during the day and day.