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本文报导使用Novozym234酶从光木耳(Auricularia auricula)和毛木耳(Auricularia polytricha)单核菌丝体中,成功地分离出高产量的原生质体;一些因素对原生质体形成的影响及原生质体再生过程中形态学变化的观察结果。菌体的液体培养时间、缓冲液pH值和一定浓度的酶液用量与原生质体的形成有着密切的关系。原生质体的两种再生型能在液体的CYM(Complete yeast extract medium)和固体的CYM含有0.6M山梨醇的再生培养基中观察到。
This article reports the successful isolation of protoplasts of high yield from Novozym aureus and Auricularia polytricha mononuclear mycelia using Novozym 234 enzyme; the effect of some factors on protoplast formation and protoplast regeneration Morphological changes in the observation. The bacterial culture liquid time, the pH value of the buffer solution and the concentration of the enzyme solution are closely related to the protoplast formation. Both regenerants of protoplasts were observed in regeneration medium containing CYM (Complete yeast extract medium) and solid CYM containing 0.6 M sorbitol.