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目的常规去溶剂法制备的麦醇蛋白颗粒因粒径偏大(400~500 nm)易被网状内皮系统捕获。探讨采用反相微乳液法制备粒径<100nm的麦醇蛋白纳米颗粒可行性。方法用反相微乳液法,以液体石蜡为油相,二硫苏糖醇(DTT)还原的麦醇蛋白乙醇溶液为水相,Tween80-Span80为复合乳化剂。按质量比1/7~1/3称取Tween80和Span80,按质量比1/6~1/2与石蜡混合均匀,配制复合乳化剂和油相的混合溶液。在水相液滴中,麦醇蛋白被加入的H2O2氧化形成二硫键而交联固化,形成纳米颗粒。结果反相微乳液法制备工艺与复合乳化剂配比、复合乳化剂与油相质量比、搅拌速度有关,得到最佳制备工艺为:以液体石蜡为油相,5.0 mg/mL麦醇蛋白乙醇/水(70%,v/v)溶液为水相,Tween80-Span80(1/7,w/w)为复合乳化剂,乙醇为助乳化剂,水相/油相比例2/30(v/w),复合乳化剂/油相比例1/3(w/w),搅拌速度1 000 r/min。得到麦醇蛋白纳米颗粒粒径为20~100 nm,颗粒中不含DTT、复合乳化剂和H2O2,纳米颗粒的热稳定性优于麦醇蛋白原料,并且纳米颗粒无细胞毒性,且细胞毒性评级均为0级。结论用反相微乳液法可以获得用作为药物载体的麦醇蛋白纳米颗粒。
Purpose Conventional desolventizing of gliadin particles is easily captured by the reticuloendothelial system because of their large particle size (400-500 nm). To investigate the feasibility of using reverse microemulsion to prepare gliadin nanoparticles with particle size <100nm. Methods The reverse phase microemulsion method, liquid paraffin as the oil phase, dithiothreitol (DTT) reduced alcohol solution of wheat protein as the aqueous phase, Tween80-Span80 as a composite emulsifier. Weigh Tween80 and Span80 according to the mass ratio of 1/7 to 1/3, and mix them evenly with paraffin at the mass ratio of 1/6 to 1/2, to prepare a mixed solution of the composite emulsifier and the oil phase. In the water phase droplets, gliadin is oxidized by the addition of H2O2 to form disulfide bonds and cross-link and solidify to form nanoparticles. Results The preparation process of reversed microemulsion and the ratio of compound emulsifier, the ratio of emulsifier to the mass ratio of oil phase and the stirring speed were the best. The optimum preparation conditions were as follows: liquid paraffin as oil phase, 5.0 mg / mL gliadin / Water (70%, v / v) as the aqueous phase, Tween80-Span80 (1/7, w / w) as the composite emulsifier and ethanol as the co-emulsifier. w), compound emulsifier / oil phase ratio 1/3 (w / w), stirring speed 1000 r / min. Obtained gem protein nanoparticles size of 20 ~ 100 nm, the particles do not contain DTT, complex emulsifier and H2O2, the thermal stability of the nanoparticles is superior to the gliadin raw material, and the nanoparticles are non-cytotoxic, and the cytotoxicity rating All levels are 0 Conclusion The use of inverse microemulsion method can be used as a drug carrier of gliadin nanoparticles.