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为了评价壳聚糖对蝴蝶兰幼苗耐热性的诱导效应,分别采用0(以无壳聚糖为对照)、25、50、100、200和400 mg·L~(-1)壳聚糖溶液处理蝴蝶兰幼苗,并置于42℃下胁迫3 d,测定了蝴蝶兰幼苗的生理指标变化。结果表明:当壳聚糖浓度由25 mg·L~(-1)增大到100mg·L~(-1)时,蝴蝶兰幼苗叶片的SOD、POD、CAT活性、脯氨酸、可溶性糖、叶绿素和类胡萝卜素含量不断增加,而质膜透性和丙二醛含量不断下降,但可溶性蛋白含量没有显著变化;特别是在100 mg·L~(-1)时,SOD、POD和CAT活性、脯氨酸、可溶性糖、叶绿素和类胡萝卜素含量增加到最大值且极显著高于对照,而质膜透性和丙二醛含量下降至最小值且极显著低于对照,蝴蝶兰幼苗遭受了轻微的高温伤害;随着壳聚糖浓度由200 mg·L~(-1)增大至400mg·L~(-1),SOD、POD和CAT活性、脯氨酸、可溶性蛋白、可溶性糖、叶绿素和类胡萝卜素含量极显著下降,而质膜透性和丙二醛含量极显著上升,蝴蝶兰幼苗的受害趋于加重;由此可见,100 mg·L~(-1)壳聚糖处理能显著提高蝴蝶兰幼苗的耐热性。
In order to evaluate the induction effect of chitosan on the heat tolerance of Phalaenopsis seedlings, the effects of chitosan on chitosan (25, 50, 100, 200 and 400 mg · L -1) The phalaenopsis seedlings were treated and placed under 42 ℃ stress for 3 days to determine the physiological changes of Phalaenopsis. The results showed that when the concentration of chitosan was increased from 25 mg · L -1 to 100 mg · L -1, the activity of SOD, POD and CAT, the content of proline, soluble sugar, The contents of chlorophyll and carotenoid increased while the contents of plasma membrane and malondialdehyde decreased, but the content of soluble protein did not change significantly. Especially at 100 mg · L -1, the activities of SOD, POD and CAT , Proline, soluble sugar, chlorophyll and carotenoid content increased to the maximum and extremely significantly higher than the control, while the plasma membrane permeability and MDA content decreased to a minimum and extremely significantly lower than the control, Phalaenopsis seedlings suffered Slight increase in temperature and high temperature. With the increase of chitosan concentration from 200 mg · L -1 to 400 mg · L -1, the activities of SOD, POD and CAT, proline, soluble protein, soluble sugar , The content of chlorophyll and carotenoid significantly decreased, while the permeability of plasma membrane and the content of malondialdehyde increased significantly, and the damage of Phalaenopsis seedlings tended to aggravate. Thus, 100 mg · L -1 chitosan Treatment can significantly improve the heat resistance of Phalaenopsis seedlings.