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目的探讨静电纺丝技术制备氧化锌/聚乙烯醇(zinc oxide/polyvinyl alcohol,ZnO/PVA)纳米纤维作为新型创伤敷料的可行性,考察其性能及对创伤愈合的促进作用。方法以溶液-凝胶法制备氧化锌纳米粒,再通过静电纺丝技术制备载有氧化锌纳米粒(1%、3%、5%)的ZnO/PVA纳米纤维并对其进行交联,采用扫描电镜和透射电镜对氧化锌纳米粒和纳米纤维进行观察,同时检测ZnO/PVA纳米纤维的透气性和吸水率,考察ZnO/PVA纳米纤维对金黄色葡萄球菌的抑制作用和对人皮肤成纤维细胞的毒性;建立大鼠皮肤创面模型,观察ZnO/PVA纳米纤维对创面的愈合作用。结果氧化锌纳米粒粒径为(64.69±20.47)nm,ZnO/PVA纳米纤维直径为(324.22±144.80)nm,纳米纤维直径较细且较为均一,交联效果明显;ZnO/PVA纳米纤维的透气性和吸水率均优于无纺布,差异具有统计学意义(P<0.05);ZnO/PVA纳米纤维对金葡萄的抑制作用较强;1%的ZnO/PVA纳米纤维无细胞毒性;ZnO/PVA纳米纤维能够促进创伤愈合,其促愈合作用优于创可贴(P<0.05)。结论采用静电纺丝技术制备的ZnO/PVA纳米纤维透气性好,吸水性强,抑菌作用较强,作用安全,能够促进创伤的愈合,有望成为理想的新型创伤敷料。
OBJECTIVE To investigate the feasibility of electrospinning zinc oxide / polyvinyl alcohol (ZnO / PVA) nanofibers as a new type of wound dressing and to investigate its performance and its promotion on wound healing. Methods ZnO / PVA nanofibers containing ZnO nanoparticles (1%, 3%, 5%) were prepared by the solution-gel method and electrospun into ZnO / PVA nanofibers. Scanning electron microscopy and transmission electron microscopy were used to observe the ZnO nanoparticles and nanofibers. At the same time, the permeability and water absorption of ZnO / PVA nanofibers were examined. The inhibitory effect of ZnO / PVA nanofibers on Staphylococcus aureus, The toxicity of cells was established. The rat skin wound model was established and the healing effect of ZnO / PVA nanofibers on the wound surface was observed. Results The diameter of ZnO / PVA nanofibers was (64.69 ± 20.47) nm and the diameter of ZnO / PVA nanofibers was (324.22 ± 144.80) nm. The diameters of nanofibers were smaller and more uniform, and the cross-linking effect was obvious. (P <0.05). ZnO / PVA nanofibers had a stronger inhibitory effect on gold grape; 1% ZnO / PVA nanofibers had no cytotoxicity; ZnO / PVA nanofibers can promote wound healing, and promote healing better than Band-Aid (P <0.05). Conclusion The ZnO / PVA nanofibers prepared by electrospinning have the advantages of good air permeability, strong water absorption, strong bacteriostasis and safe action, which can promote wound healing and is expected to be an ideal new wound dressing.