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背景:虽然人们对铅毒性引起机体的损害有了较深入认识,但铅负荷对T细胞淋巴细胞亚群的影响还不十分明确。目的:评估血铅对儿童T细胞亚群的影响,以及血铅临界值与T细胞亚群的关系。设计:对比观察。单位:山西省儿童医院妇幼保健院分子微生物研究所。对象:选择2003-05/2003-10太原市南郊和北郊区儿童60人,男32人,女28人;年龄3～6岁,平均(4.5±1.5)岁。监护人均对检测项目知情同意。根据血铅值将儿童分为3组:≥0.48μmol/L组(n=13),≥0.24μmol/L,<0.48μmol/L组(n=20),<0.24μmol/L组(n=27)。方法:①采用石墨炉原子吸收光谱法测定血铅水平。T细胞亚群检测用免疫荧光法。②计量资料差异性比较采用t检验。血铅与T细胞亚群关系采用直线相关分析。主要观察指标:①不同血铅水平儿童T细胞亚群表达比较。②血铅水平与T细胞亚群的关系。结果:儿童60人均进入结果分析。①≥0.48μmol/L组儿童CD3表达及CD4/CD8比值明显低于<0.24μmol/L组(t=3.27,3.07,P<0.01)。②血铅值≥0.48μmol/L时血铅水平与CD3表达及CD4与CD8比值呈显著负相关(r=-0.689,-0.674,P<0.01),与CD8表达呈显著正相关(r=0.748,P<0.01)。结论:血铅值水平≥0.48μmol/L,可使外周血CD3表达及CD4与CD8比值下降,对小儿免疫功能产生不良影响。
BACKGROUND: Although there is a deep understanding of the damage caused by lead toxicity, the effect of lead load on T cell lymphocyte subsets is not yet clear. PURPOSE: To assess the effect of blood lead on children’s T-cell subsets and the relationship between the blood lead threshold and T-cell subsets. Design: comparative observation. Unit: Institute of Molecular Microbiology, Maternal and Child Health Hospital, Shanxi Children’s Hospital. PARTICIPANTS: A total of 60 children, 32 males and 28 females, aged from 3 to 6 years (mean 4.5 ± 1.5 years), were selected from 2003-05 to 2003-10 in the southern and northern suburbs of Taiyuan City. Guardian informed consent of the test items. The children were divided into 3 groups according to their blood lead levels: ≥0.48μmol / L group (n = 13), ≥0.24μmol / L, 0.48μmol / L group (n = 27). Methods: ① Blood lead levels were determined by graphite furnace atomic absorption spectrometry. T cell subsets were detected by immunofluorescence. ② measurement data were compared using t test. The relationship between blood lead and T cell subsets was analyzed by linear correlation analysis. MAIN OUTCOME MEASURES: ① Comparison of T cell subsets expression in children with different blood lead levels. ② blood lead levels and T cell subsets relationship. Results: 60 children entered the result analysis. ① The CD3 expression and CD4 / CD8 ratio in the group with≥0.48μmol / L were significantly lower than those in the group <0.24μmol / L (t = 3.27,3.07, P <0.01). ② Blood lead levels were significantly negatively correlated with CD3 expression and CD4 / CD8 ratio (r = -0.689, -0.674, P <0.01), and positively correlated with CD8 expression (r = 0.748 , P <0.01). Conclusion: The level of blood lead level ≥0.48μmol / L, can make peripheral blood CD3 expression and the ratio of CD4 and CD8 decreased, have an adverse effect on children’s immune function.