净化是农药残留分析中前处理的重要环节,常规的步骤往往是先经液—液分配。然后进行柱层析,其缺点是手工操作多,溶剂用量大,耗费时间长。高效液相色谱法能对混合物进行有效的分离,但是检测的灵敏度较低,目前尚未普遍用于农药的残留量分析,笔者在瑞士汔巴—嘉基公司残留实验室学习期间,对杀菌剂瑞毒霉(Ridomil)的净化采用自行制备的液相色谱柱,获得较好的效果。蔬菜样本抽提后经装在有(Kontron Uvikon LCD 725)鉴定 Purification is an important part of the pretreatment of pesticide residue analysis, the conventional steps are often first liquid - liquid distribution. Then the column chromatography, the disadvantage is that more manual operation, the amount of solvent used, take a long time. High performance liquid chromatography can effectively separate the mixture, but the detection sensitivity is low, so far it has not been widely used in pesticide residues analysis. The author studied the residual bactericide Ridomil purification using self-prepared liquid chromatography column, get better results. Samples of vegetables were extracted and identified on a (Kontron Uvikon LCD 725)