论文部分内容阅读
目的:研究妊娠期糖尿病(GDM)患者血清中胰高血糖素样肽-1(GLP-1)和二肽基肽酶-IV(DPP-IV)的水平,并探讨其在妊娠期糖尿病中的作用。方法:采用ELISA法检测43例GDM患者、38例糖耐量正常孕妇及35例正常非孕妇血清GLP-1及DPP-IV的水平;同时测定3组妇女空腹血糖(FPG)、胰岛素(FINS)及糖化血红蛋白(HbA1c)的水平,并根据HOMA稳态模型提出的公式,计算胰岛素抵抗指数(HOMA-IR)和胰岛素分泌指数(HOMA-HBCI)进一步评价胰岛β细胞的功能。结果:①GDM患者血清GLP-1水平明显低于糖耐量正常孕妇组及正常非孕妇组,差异有统计学意义(P<0.05),血清DPP-IV水平明显高于糖耐量正常孕妇组及正常非孕妇组,差异有统计学意义(P<0.05);②GDM患者与另两组相比,存在明显的胰岛素抵抗(HOMA-IR明显增大,P<0.05),而胰岛素分泌明显下降(HOMA-HBCI明显减小,P<0.05),差异均有统计学意义。结论:GDM患者血清GLP-1水平明显下降,而DPP-IV水平明显升高,可能在GDM的发病中发挥一定的作用。
Objective: To investigate the levels of serum glucagon-like peptide-1 (GLP-1) and dipeptidyl peptidase-IV (DPP-IV) in patients with gestational diabetes mellitus (GDM) effect. Methods: Serum GLP-1 and DPP-IV levels were measured in 43 GDM patients, 38 normal glucose tolerance pregnant women and 35 normal non-pregnant women by ELISA. Fasting plasma glucose (FPG), insulin (FINS) The level of HbA1c was determined and the function of islet β cells was further evaluated according to the formula proposed by HOMA homeostasis model. HOMA-IR and HOMA-HBCI were calculated. Results: ① The serum GLP-1 level in patients with GDM was significantly lower than that in normal glucose tolerance group and normal non-pregnant women (P <0.05), serum DPP-IV level was significantly higher than normal glucose tolerance group and normal non-pregnant women (P <0.05); ②GDM patients had obvious insulin resistance (HOMA-IR significantly increased, P <0.05) and insulin secretion decreased significantly compared with the other two groups (HOMA-HBCI Significantly reduced, P <0.05), the differences were statistically significant. Conclusion: The serum level of GLP-1 in patients with GDM is significantly decreased, while the level of DPP-IV is significantly increased, which may play a role in the pathogenesis of GDM.