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[目的]探讨三氧化二砷(As2O3)对前列腺癌细胞株PC-3细胞增殖、细胞凋亡及X染色体连锁凋亡抑制蛋白(XIAP)表达的影响.[方法]取对数生长期PC-3细胞,分别经0.5,1.0,2.0μmol/L As2O3处理,采用四甲基偶氮唑蓝比色法检测细胞生长抑制作用;流式细胞仪检测细胞凋亡率;蛋白质印迹法及实时荧光定量PCR检测XIAP蛋白表达及XIAP mRNA表达的变化.[结果]0.5,1.0,2.0μmol/LAs2O3均可抑制PC-3细胞增殖,在处理24,48,72h后,细胞生长抑制率间差异具有统计学意义(P<0.01).检测0.5,1.0,2.0μmol/L As2O3处理48h的PC-3细胞株细胞凋亡率分别为11.47%±1.81%,38.47%±1.60%,58.93%±3.35%,相比较差异具有统计学意义(P<0.01).As2O3处理48h时0.5,1.0,2.0μmol/L As2O3组的XIAP mRNA表达水平分别为0.67±0.12,0.25±0.15,0.03±0.01,各组间差异均具有统计学意义(P<0.05).[结论]As2O3可抑制PC-3细胞增殖,诱导细胞凋亡,此作用可能与As2O3抑制PC-3细胞XIAP基因的表达有关联.
[Objective] To investigate the effects of arsenic trioxide (As2O3) on proliferation, apoptosis and expression of X-linked inhibitor of apoptosis protein (XIAP) in prostate cancer cell line PC-3. [Methods] The cells were treated with 0.5, 1.0 and 2.0μmol / L As2O3, respectively. The cell growth inhibition was detected by MTT assay; the apoptosis rate was detected by flow cytometry; Western blotting and real-time fluorescence quantitative PCR Protein expression and XIAP mRNA expression. [Results] The proliferation of PC-3 cells was inhibited by 0.5,1.0,2.0 micromol / L As2O3, and there was significant difference between the cell growth inhibition rates at 24, 48 and 72 h <0.01) .Apoptotic rates of PC-3 cells treated with 0.5, 1.0, 2.0μmol / L As2O3 for 48h were 11.47% ± 1.81%, 38.47% ± 1.60% and 58.93% ± 3.35% (P <0.01) .At 48 hours after treatment, the mRNA expression levels of XIAP in 0.5,1.0,2.0μmol / L As2O3 group were 0.67 ± 0.12,0.25 ± 0.15,0.03 ± 0.01, the differences between groups were statistically significant (P <0.05). [Conclusion] As2O3 can inhibit the proliferation of PC-3 cells and induce the apoptosis of cells, which may be related to As2O3 inhibiting the expression of XIAP gene in PC-3 cells Of association.