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目的利用CDDP标记技术对43份铁皮石斛Dendrobium officinale野生和栽培种质进行初步抗性筛选和遗传多样性分析,为铁皮石斛产业以及优良品种筛选保护提供理论基础。方法从21条CDDP引物中筛选出产物清晰、多态性好的引物对供试材料基因组DNA进行扩增。结果 16条引物共扩增出了151条带,其中多态性条带144条,多态性比率95.7%,表明供试材料具有丰富的遗传多样性。种群遗传多样性结果表明,3个种群多态性位点比例(PPL)为65.56%~82.12%,种群间基因分化系数(Gst)为0.110 2,基因流(Nm)为4.035 4,说明人为选择亦可能促进铁皮石斛的遗传多样性。结论铁皮石斛人工栽培种具有丰富的遗传多样性,同时统计结果分析表明,43份材料中可以初步筛选出8份具有潜在优良抗性的植株。
OBJECTIVE: To screen and characterize 43 wild Dendrobium officinale wild and cultivated germplasms using CDDP marker technology, and to provide a theoretical basis for the screening and protection of Dendrobium candidum industry. Methods A total of 21 CDDP primers were screened for their clear and polymorphic primers to amplify the genomic DNA of the tested materials. Results A total of 151 bands were amplified with 16 primers, of which 144 were polymorphic bands with a polymorphic ratio of 95.7%, which indicated that the tested materials were rich in genetic diversity. The result of population genetic diversity showed that the PPL of three populations ranged from 65.56% to 82.12%, the coefficient of genetic differentiation (Gst) between populations was 0.110 2, and the gene flow (Nm) was 4.035 4, which indicated that human selection It may also promote the genetic diversity of D. officinale. Conclusion Artificial cultivars of Dendrobium candidum are rich in genetic diversity. Statistical analysis shows that eight of the 43 cultivars can be initially screened for potential excellent resistance.