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通过一系列的试验,确定了最佳的凝胶色谱试验条件:采用含有0.025 mol/L KNO_3的DMF为流动相;样品质量浓度为1 mg/m L,进样量200μL;柱温采用80℃,采用窄分布的聚苯乙烯为标样绘制标准曲线,然后采用纯PAN标样校正仪器。该方法快速准确,具有良好的重现性,可满足生产和科研的需要。
Through a series of experiments, the best conditions of gel chromatography were determined: using DMF containing 0.025 mol / L KNO 3 as the mobile phase; the mass concentration of the sample was 1 mg / m L and the injection volume was 200 μL; the column temperature was 80 ℃ , Using a narrow distribution of polystyrene as a standard curve to draw a standard curve, and then use a pure PAN standard calibration equipment. The method is fast and accurate, has good reproducibility, and can meet the needs of production and scientific research.