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近年来,国外广泛应用生物学方法(生物指示剂)来检查湿热灭菌过程的有效性。该生物指示剂中的耐热微生物是嗜热脂肪芽胞杆菌(Bacillusstearothermophilus)的芽胞,并要求在生物指示剂中的芽胞数量应超过被灭菌物品上带有的微生物数量的通常水平。而用于生物指示剂的培养基则要求它在加热过程中能保护芽胞不致死亡,并能促进经受过加热的芽胞生长,以及在指示剂保存期芽胞可以存活。用于生物指示剂的pH指示剂则要求其
In recent years, extensive use of biological methods abroad (biological indicator) to check the effectiveness of wet heat sterilization process. The heat-resistant microorganism in the biological indicator is a spore of Bacillus stearothermophilus and requires that the number of spores in the biological indicator should exceed the usual level of the number of microorganisms carried on the sterilized article. The medium used for the biological indicator, on the other hand, requires that it protect the spores from death during the heating process, and promote the growth of the heated spores, and the spores may survive during the indicator storage period. PH indicators for biological indicators require it