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合成了新配体２－苯基（４－氯）咪唑［ｆ］邻菲咯啉（简称ＰＩＰ（Ⅱ），并合成络合物Ｒｕ（ｂｐｙ）_２ＰＩＰ（Ⅲ），研究了该络合物与ＤＮＡ作用的荧光光谱，观察到５９０ ｎｍ（λ_ｅｘ＝４６０ｎｍ）处产生很强的荧光峰，采用流动注射法测定ＤＮＡ，该方法简便快速，灵敏度高。对合成样品测定获得满意结果。
A new ligand, PIP (Ⅱ), was synthesized and the complex Ru (bpy) _2PIP (Ⅲ) was synthesized. The complex of DNA fluorescence spectra, the observed strong fluorescence peak at 590 nm (λ_ex = 460nm), the flow injection method for the determination of DNA, the method is simple and rapid, high sensitivity. Determination of synthetic samples obtained satisfactory results.