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目前测定异烟肼的碘滴定法或美国药典(十八版)测定片剂中异烟肼的方法既费时又麻烦。本文报导一个简便且灵敏度高的比色法。本法是基于异烟肼在硼砂的无水甲醇溶液中与7-氯-4-硝基苯并-2-氧杂-1,3-二氮杂茂相互作用,产生的橙色物可在467毫微米处测定其吸收值。反应方程为: 异烟肼在0.3～5微克/毫升的浓度范围内,符合Beer-Lambert定律。在90分钟内显色稳定。测定9个含2微克/毫升的异烟肼样品的重演性,其标准偏差为0.87%。如测定过程中有水存在则重演性不佳。用本法测定片剂和糖浆中的异烟肼,并与美国药典(十八版)的方法作了比较,结果
The current method of iodometric titration of isoniazid or of the United States Pharmacopeia (eighteenth edition) for the determination of isoniazid in tablets is both time-consuming and cumbersome. This article reports a simple and highly sensitive colorimetric method. This method is based on the interaction of isoniazid with 7-chloro-4-nitrobenz-2-oxa-1, 3-diazepine in anhydrous boranes in methanolic solution resulting in an orange color at 467 Absorbance was measured at nm. The reaction equation is: isoniazid in the 0.3 ~ 5 micrograms / ml concentration range, in line with Beer-Lambert’s law. Color stable within 90 minutes. Reproducibility of 9 isoniazid samples containing 2 μg / mL was determined with a standard deviation of 0.87%. Such as the determination of the existence of water is poor reproducibility. Determination of isoniazid in tablets and syrups by this method was compared with that of the U.S. Pharmacopeia (eighteenth edition) and the results