论文部分内容阅读
目的:探讨大气可吸入颗粒物(PM10)对大鼠鼻黏膜的急性损伤作用及其可能的机制。方法:分别使用高、中、低3个剂量的PM10给予大鼠鼻腔内染毒1周。苏木精-伊红染色及扫描电镜下观察染毒对鼻黏膜形态学的影响。结果:苏木精-伊红染色后显示PM10可导致大鼠鼻黏膜上皮细胞脱落,杯状细胞增生,固有层水肿,炎性细胞浸润,腺体增生,腺管狭窄堵塞,血管扩张出血。3个染毒剂量组与生理盐水组炎性细胞计数比较均差异有统计学意义(均P<0.05),黏膜结构损伤的半定量记分高、中剂量组与生理盐水组比较均差异有统计学意义(均P<0.05)。以上改变均存在剂量-反应关系。扫描电镜下显示PM10可导致大鼠鼻黏膜上皮纤毛稀疏、脱落、倒伏,集结缠绕,分泌物黏附。结论:大气可吸入颗粒物对大鼠鼻黏膜具有损伤和致炎症作用。
Objective: To investigate the acute injury of nasal mucosa by inhalable particles (PM10) and its possible mechanism. Methods: Three doses of PM10 were administered to rats for one week. Effects of Exposure to Morphology of Nasal Mucosa with Hematoxylin - Eosin Staining and Scanning Electron Microscopy. Results: Hematoxylin - eosin staining showed that PM10 could lead to the loss of epithelial cells of the nasal mucosa, goblet cell hyperplasia, edema of the lamina propria, infiltration of inflammatory cells, hyperplasia of the glandular duct, blockage of the ductal stenosis and vasodilation. There were significant differences in the inflammatory cell counts between the three exposure dose groups and the saline group (all P <0.05), and the semi-quantitative scoring of mucosal injury was higher than that of the saline group Significance (all P <0.05). The above changes are dose-response relationship. Scanning electron microscopy showed that PM10 could cause sparse, detached, lodging, convoluted wounds and secretions adhesion of nasal mucosa epithelium in rats. Conclusion: Inhalable particles of the atmosphere can damage the rat nasal mucosa and cause inflammation.