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目的:观察益气健脾法代表方剂对实验性脾虚证大鼠模型红细胞膜腺苷三磷酸酶(ATPase)活性的影响。方法:取SD大鼠60只随机分为6组,即正常对照组、脾虚模型组、四君子汤组、当归补血汤组、黄芪四君子汤组、当归补血汤合四君子汤组,采用破气苦降加饥饱失常法复制脾虚证大鼠模型,用定磷法检测大鼠红细胞膜ATPase的活性。结果:益气健脾方药能显著升高脾虚证大鼠模型红细胞膜ATPase的活性。结论:从微观的角度验证了中医“脾主运化”的理论。
Objective: To observe the effect of Yiqi Jianpi recipe on erythrocyte membrane ATPase activity in experimental Spleen Deficiency Syndrome rats. Methods: Sixty SD rats were randomly divided into 6 groups: normal control group, spleen deficiency model group, Sijunzitang group, Dangguibuxue decoction group, Astragalus Sijunzi Decoction group, Danggui Buxue decoction combined with Sijunzi decoction group, The model of spleen deficiency syndrome was duplicated with the method of starvation and insufficiency, and the ATPase activity of erythrocyte membrane was detected by the method of fixed phosphorus. Results: Yiqi Jianpi Recipe could significantly increase the activity of erythrocyte membrane ATPase in rats with spleen deficiency syndrome. Conclusion: From the microscopic point of view, the theory of traditional Chinese medicine “spleen transport” has been verified.