本文对浓集和分离约氏疟原虫约氏亚种的方法进行了探讨。应用17.5％的聚蔗糖(Ficoll)溶液可浓集该种疟原虫感染的红细胞,并可分离出以裂殖体为主的上层和滋养体占50％左右的中层感染细胞层。应用ConA-Sepharose 4B柱可以分离出大量比较纯净的裂殖子,其形态完整,纯度在90％以上。 In this paper, the methods of concentration and isolation of Plasmodium yoelii Y. subsp. The 17.5% solution of Ficoll was used to concentrate the erythrocytes infected with Plasmodium, and 50% of the infected middle and upper trophozoites with schizonts were isolated. A large number of relatively pure merozoites can be isolated using ConA-Sepharose 4B column, which has a complete morphology with a purity of over 90%.