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[目的]探讨半夏泻心汤加减方调控热休克蛋白27(HSP27)干预乙酸性胃溃疡(GU)大鼠的疗效机制。[方法]30只Wista大鼠,随机分为假手术对照(S)组、GU模型对照(M)组及半夏泻心汤加减方干预(Y)组,每组10只。M及Y组采用胃窦部乙酸注射复制大鼠乙酸性GU模型,S组注射同剂量的0.85%氯化钠。于术后第2天Y组经灌胃給予半夏泻心汤加减方每日1次,共7 d,S、M组给予等量0.85%氯化钠,于术后第8天处死全部大鼠取材,观察胃大体情况,采用GU指数(GUI)对溃疡进行评定,苏木精-伊红染色观察胃组织病理变化,实时荧光定量PCR法检测大鼠胃窦部HSP27 mRNA表达。[结果]S组大鼠胃腔无溃疡形成,M及Y组大鼠胃腔乙酸注射部位均见溃疡形成,但Y组GUI显著低于M组(P<0.01);Y组胃组织HSP27mRNA较S、M组表达显著升高(均P<0.01)[结论]半夏泻心汤加减方可上调HSP27mRNA表达,这可能是其有效干预乙酸性GU大鼠的病理机制。
[Objective] To explore the therapeutic mechanism of Banxia Xiexin Decoction plus or minus side in the regulation of heat shock protein 27 (HSP27) intervention in acetic acid gastric ulcer (GU) rats. [Methods] Thirty Wistar rats were randomly divided into sham-operated control (S) group, GU model control (M) group and Pinellia Xiexin Decoction plus intervention group (Y), 10 in each group. Rats in groups M and Y were treated with acetic acid injection of acetic acid, and rats in group S were injected with the same dose of 0.85% sodium chloride. On the second postoperative day, the Y group was given intragastric administration of Banxia Xiexin Decoction once a day for 7 days, and the S and M groups were given an equal volume of 0.85% sodium chloride, and all of them were sacrificed on the 8th day after operation. Rats were selected to observe the general condition of the stomach. Ulcers were evaluated using GU index (GUI). Pathological changes of gastric tissue were observed with hematoxylin-eosin staining. HSP27 mRNA expression in gastric antrum was detected by real-time fluorescence quantitative PCR. [Results] In the S group, there was no ulcer formation in the stomach cavity, and ulceration was found in the acetic acid injection sites in the rats of the M and Y groups. However, the GUI of the Y group was significantly lower than that of the M group (P<0.01); the HSP27 mRNA of the stomach tissue of the Y group was comparable The expression of HSP27 mRNA was significantly increased in S and M groups (all P<0.01). [Conclusion] The addition of Bixia Xiexin Decoction can up-regulate the expression of HSP27 mRNA, which may be the mechanism of its effective intervention in acetic acid-induced GU rats.