目的研究B erland iera lya tra中的抗肿瘤活性化学成分。方法以M TT法进行抗肿瘤活性化学成分的追踪,利用硅胶柱色谱和Sephadex-LH-20分离化合物,用理化和光谱数据确定化合物结构。结果从B erland iera lya-tra的CHC l3活性部位中,分离得到了两个细胞毒活性化合物,并确定其为3α-环氧短小伯兰菊素(3-αepoxypum ilin)和短小伯兰菊素(pum ilin)。结论3α-环氧短小伯兰菊素和短小伯兰菊素是该植物抗肿瘤的主要活性成分,也是首次从该植物中分离得到的抗肿瘤活性成分。 Objective To study the chemical components of anti-tumor activity in Berland iera lya tra. Methods The chemical components of antitumor activity were tracked by M TT method. The compounds were isolated by silica gel column chromatography and Sephadex-LH-20. The physicochemical and spectral data were used to determine the structure of the compounds. Results Two active compounds of cytotoxicity were isolated from the active site of CHC 13 in Berland iera lya-tra and identified as 3α-epoxypum ilin and cyanide (pum ilin). Conclusion The 3α-epoxy-short-cremperin and short-creverin is the main anti-tumor active components of the plant, and is the first anti-tumor active component isolated from this plant.