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以苹果悬浮细胞为试材,以硝普钠(SNP)为一氧化氮(NO)供体,L-NNA(Nx-nitro-L-ar-ginine)为一氧化氮合酶(NOS)抑制剂,PTIO(2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide)为NO清除剂,研究了茉莉酸甲酯(MeJA)处理苹果悬浮细胞诱发NO和H2O2信号分子及其对PAL酶活性、植保素和丙二醛(MDA)的影响。结果表明,MeJA处理能明显诱发NO生成,在处理6h时NO生成量达到峰值,而L-NNA和PTIO则能够抑制NO生成。另外,MeJA处理也显著提高了细胞内的H2O2水平,而SNP单独处理不能提高H2O2水平。在各种处理中,PAL活性以及植保素和MDA含量均表现出先升后降趋势,分别在4h或6h时达到峰值。本研究结果揭示了MeJA可以激发NO生成,而细胞内H2O2水平的提高则是MeJA和NO协同作用的结果,植保素和MDA含量以及PAL活性的提高主要受NO的诱导,但也存在其它诱导途径。
Using suspension cells of apple as experimental material, nitric oxide (NO) donor was served as SNP, and nitric oxide synthase (NOS) inhibitor was used as N-nitro-L-arginine , PTIO (2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide) as the NO scavenger, the effects of methyl jasmonate (MeJA) Molecules and their effects on PAL activity, phytoalexin and malondialdehyde (MDA). The results showed that MeJA treatment significantly induced NO production, reached its peak at 6h treatment, while L-NNA and PTIO inhibited NO production. In addition, MeJA treatment also significantly increased intracellular H2O2 levels, whereas SNP alone did not increase H2O2 levels. In all treatments, PAL activity and phytoalexins and MDA contents increased first and then decreased, reaching the peak at 4h or 6h. The results of this study revealed that MeJA stimulated the production of NO, while the increase of intracellular H2O2 was the result of the synergistic effect of MeJA and NO. The increase of phytoalexin and MDA content and PAL activity were mainly induced by NO, but other induction pathways existed .