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目的:确定BED捕获酶联免疫试验(BED法)检测发现HIV新近感染者的灵敏度和特异度。方法:以云南省德宏州感染时间>168 d的HIV感染者为“真正的”既往感染者,以感染时间≤168 d的HIV感染者为“真正的”新近感染者,对其血清标本采用BED法进行检测。结果:29例新近感染者中BED检测阳性共24例,灵敏度为82.76%;264例既往感染者中BED检测阴性共218例,特异度为82.58%;感染者感染时间越长,BED特异度越高,而对于感染时间在169 d～365 d的感染者,BED特异度低、假阳性率高。结论:BED法在确定HIV新近感染者时具有较高的灵敏度和特异度,但对部分感染者存在一定的误判率。
OBJECTIVE: To determine the sensitivity and specificity of the BED Trap ELISA (BED method) to detect newly infected HIV. Methods: HIV-infected persons who were infected for more than 168 days in Dehong Prefecture of Yunnan Province were considered as “genuine” former HIV-infected persons and those newly infected with “true” HIV-infected persons whose infection time was less than or equal to 168 days The serum samples were detected by BED method. Results: Among the 29 newly infected patients, 24 cases were positive for BED test, the sensitivity was 82.76%. Among 264 cases of previous infection, 218 cases were negative for BED test and the specificity was 82.58%. The longer the infection was, the more the BED was High, while for infection in 169 d ~ 365 d of infection, BED low specificity, false positive rate. Conclusion: The BED method has high sensitivity and specificity in determining newly infected HIV patients, but there is a certain misjudgment rate for some infected patients.